Fluid Lipid Multilayer Stabilization by Tetraethyl Orthosilicate for Underwater AFM Characterization and Cell Culture Applications
| Autor: | Nicholas Vafai, Troy W. Lowry, Steven Lenhert, David H. Van Winkle, Aubrey Kusi-Appiah |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Atomic force microscopy Mechanical Engineering Analytical chemistry Biomaterial 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences Characterization (materials science) Tetraethyl orthosilicate chemistry.chemical_compound Chemical engineering chemistry Mechanics of Materials Cell culture Liquid crystal medicine lipids (amino acids peptides and proteins) General Materials Science Swelling medicine.symptom 0210 nano-technology |
| Zdroj: | MRS Advances. 2:3553-3558 |
| ISSN: | 2059-8521 |
| DOI: | 10.1557/adv.2017.502 |
| Popis: | Stabilization of surface supported fluid lipid multilayers for underwater characterization is an essential step in making them useful for scalable cell culture applications such as high throughput screening. To this end, we used tetraethyl orthosilicate (TEOS), recently shown to stabilize fluid lipid films while maintaining their fluidity and functionality under water, to stabilize lipid multilayer micropatterns of 1, 2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). The treated multilayers were immersed under water and successfully imaged by atomic force microscopy (AFM), a difficult feat to perform on fluid lipid multilayers without TEOS treatment. The treated lipid multilayer showed an average swelling of approximately 18% in water but remained stable during the imaging process. The TEOS-treated lipid multilayers also proved compatible with cell culture as HeLa, MDCK, and HEK cell types all adhered and grew in high numbers over the multilayers. The results obtained here open the door to the use of fluid lipid multilayers in biotechnology applications such as microarray based high throughput cell assays. |
| Databáze: | OpenAIRE |
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