| Autor: |
Pauline Brousseau, Yves Payette, Helen Tryphonas, Barry Blakley, Herman Boermans, Denis Flipo, Michel Fournier, Martin Beaudet, Edouard Kouassi, Patrice Lapierre, Isabelle Voccia |
| Rok vydání: |
2021 |
| Předmět: |
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| Popis: |
Identification, Anaesthesia, and Euthanasia Identification of Laboratory Rodents by Ear Notching Identification of Fish by Fin Clipping Anaesthesia of Fish and Rodents Euthanasia of Rodents Euthanasia of Fish Working Sheet: Weight of Laboratory Animals Collection of Peripheral Blood Samples Reagents Materials and Equipment Procedure Removal of Organs Reagents Materials and Equipment Procedure Preparation of Cell Suspensions Extrusion of Coelomocytes in the Earthworm Hemocyte Collection in the Mollusk Cell Suspension from Peritoneal Exudate Isolation of White Blood Cells from Peripheral Blood Cell Suspensions from Lymphoid Organs Assessment of Cell Viability Determination of Cell Viability and Concentration by Trypan Blue Dye Exclusion Determination of Cell Viability and Cell Concentration with Ethidium Bromide and Acridine Orange Flow Cytometric Assessment of Cell Viability Working Sheet: Cell Viability and Concentration by Trypan Blue Dye Exclusion or Ethidium Bromide/Acridine Orange Cell Cryopreservation Reagents Materials and Equipment Procedure Special Recommendations Analysis of Results Species-Related Changes in the Protocol Technique-Related Changes in the Protocol: Phagocytosis with Yeast Statistical Tests Working Sheet: Phagocytic Function Oxidative Burst Assay Using Flow Cytometry Reagents Materials and Equipment Procedure Special Recommendations Analysis of Results Species-Related Changes in the Protocol Statistical Tests Working Sheet: Acquisition List Cell Cytotoxicity Natural Killer (NK) Cell Activity Antibody-Dependent Cellular Cytotoxicity Lymphokine-Activated Killer (LAK) Working Sheet: Acquistion List Lymphoblastic Transformation Reagents Materials and Equipment Procedure Analysis of Result Special Recommendations Species-Related Changes in the Protocol Working Sheet: Mitogenic Assay Determination of Antibody-Producing Cells to a Specific Antigen Liquid Plaque Forming Cell Assay in Mouse Model Agar Plaque Forming Cell Assay in Rat Model Working Sheet: Plaque Forming Cells (PFC) Mixed Lymphocyte Reaction (MLR) Reagents Materials and Equipment Procedure Analysis of Results Special Recommendations Species-Related Changes in the Protocol Working Sheet: Mixed Lymphocyte Reaction Intracellular Levels of Calcium Assay Using Flow Cytometry Reagents Materials and Equipment Procedure Special Recommendations Analysis of Results Species-Related Changes in the Protocol Statistical Tests Working Sheet: Acquisition List Phenotyping of Blood Mononuclear Cells Reagents Materials and Equipment Procedure Special Recommendations Analysis of Results Species-Related Changes in the Protocol Immunophenotype of Rat Peripheral Blood Lymphocytes Working Sheet Evaluation of Intracellular Level of Thiols Reagents Materials and Equipment Procedure Special Recommendations Analysis of Results Species-Related Changes in the Protocol Working Sheet: Acquisition List Apoptosis Reagents Materials and Equipment Procedure Analysis of Results |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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