Selective retrieval of microparticles in microchambers using electrolytically generated bubbles for cell array applications
| Autor: | Tomoaki Kurakazu, Yasuyuki Sakai, Teruo Fujii, Shoji Takeuchi, Kaori Kuribayashi-Shigetomi, Hiroshi Kimura, Yukiko T. Matsunaga |
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| Rok vydání: | 2011 |
| Předmět: |
Electrolysis
Materials science Cellular array Metals and Alloys Spheroid Nanotechnology Condensed Matter Physics Cellular viability Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention Indium tin oxide chemistry.chemical_compound chemistry law Electrode Materials Chemistry Polystyrene Electrical and Electronic Engineering Instrumentation |
| Zdroj: | Sensors and Actuators B: Chemical. 159:229-233 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2011.06.077 |
| Popis: | This paper describes a method for the selective retrieval of microparticles using bubbles generated by electrolysis. Microparticles (polystyrene beads, mouse embryos, and cell spheroids) were arrayed in microchambers fabricated in SU-8 on the surface of an electrode consisting of indium tin oxide (ITO) patterned on glass. Bubbles were selectively generated in a target microchamber by applying a voltage to electrodes positioned in the microchamber. As a result, we successfully retrieved microparticles (100 μm in diameter) positioned in the microchambers. This method is gentle enough to maintain cellular viability, and therefore, it will be a powerful tool for the quantitative analysis of cells in an arrayed system. |
| Databáze: | OpenAIRE |
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