Conversion of hydrolysates of corn cobs and hulls into ethanol by recombinantEscherichia coli B containing integrated genes for ethanol production

Autor:	R. G. Hall, D. E. Fowler, Beall David S, Arie Ben-Bassat, Joy B. Doran, B. E. Wood, Lonnie O. Ingram
Rok vydání:	1992
Předmět:	Ethanol Starch food and beverages Bioengineering General Medicine Applied Microbiology and Biotechnology Wet-milling Hydrolysate chemistry.chemical_compound chemistry Biochemistry Ethanol fuel Hemicellulose Fermentation Food science Sugar Biotechnology
Zdroj:	Biotechnology Letters. 14:857-862
ISSN:	1573-6776 0141-5492
Popis:	Hemicellulose and residual starch in corn hulls from wet milling and hemicellulose in corn cobs were hydrolyzed by incubation in dilute sulfuric acid at 140°C to 160°C. These hydrolysates were efficiently fermented to ethanol by a genetically engineered derivative ofE. coli B, strain KO11. Fermentation of com hull hydrolysate was complete after 48 h with a final ethanol concentration of 38 grams per liter. Fermentation of corn cob hydrolysate was essentially complete after 24 h due to a lower concentration of sugars and higher levels of inocula. In both cases, ethanol produced was equivalent to 100% of the maximum theoretical yield (0.51 grams ethanol/gram sugar) based on momoner sugar content. ThusE. coli B strain KO11 appears to be an excellent candidate for the efficient production of ethanol from hydrolysates of corn residues.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f3e60adf6c5c190451d0d71837fa4ff2 https://doi.org/10.1007/bf01029153 Zobrazit plný text záznamu Full text from SpringerLink