Thermo-Magnetic Properties of Fe3O4@Poly(N-Isopropylacrylamide) Core–Shell Nanoparticles and Their Cytotoxic Effects on HeLa and MDA-MB-231 Cell Lines
Autor: | Reynaldo Esquivel, Armando Lucero-Acuña, Enrique Álvarez-Ramos, Pedro Hernández, Iván E. Moreno-Cortez |
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Rok vydání: | 2020 |
Předmět: |
Materials science
Nanocomposite Biomedical Engineering Nanoparticle Infrared spectroscopy Bioengineering 02 engineering and technology General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Lower critical solution temperature chemistry.chemical_compound Chemical engineering Dynamic light scattering chemistry Scanning transmission electron microscopy Poly(N-isopropylacrylamide) General Materials Science 0210 nano-technology Superparamagnetism |
Zdroj: | Journal of Nanoscience and Nanotechnology. 20:2063-2071 |
ISSN: | 1533-4880 |
DOI: | 10.1166/jnn.2020.17324 |
Popis: | In this study, we performed a systematic evaluation of the synthesis parameters of a multiresponsive core-shell nanoplatform (Fe₃O₄ nanoparticles coated by poly(N-isopropylacrylamide). These nanocomposites allow the possibility of controlling specific properties, such as particle size and morphology. The polymer coating was performed by in-situ free-radical polymerization of N-isopropylacrylamide on Fe₃O₄ nanoparticles to obtain a nanocomposite with a core-shell structure. We monitored the size and morphology of the nanocomposite by scanning transmission electron microscopy. Electrophoretic mobility determined the evolution of ζ-potentials during coating. We assessed the functionalization of the Fe₃O₄ nanoparticles by Fourier-transform infrared spectroscopy and ultraviolet-visible spectroscopy. The core-shell systems exhibited a collapsed structure when heated above the lower critical solution temperature, which was determined by dynamic light scattering. Squid based vibrating sample magnetometer tests were performed to verify superparamagnetic behavior at room temperature. The platform was approved as biocompatible for the HeLa and MDA-MB-231 cell lines by MTT assays, and it shows the desired properties for potential use in localized and controlled drug delivery. |
Databáze: | OpenAIRE |
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