Impaired calcium signaling in muscle fibers from intercostal and foot skeletal muscle in a cigarette smoke-induced mouse model of COPD
| Autor: | Shyam Biswal, Martin F. Schneider, Thomas E. Sussan, Hegang Chen, Erick O. Hernández-Ochoa, Patrick Robison |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Calmodulin Physiology Biology 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Physiology (medical) Internal medicine medicine Respiratory muscle Respiratory system Calcium signaling COPD Skeletal muscle Anatomy medicine.disease Pathophysiology 030104 developmental biology Endocrinology medicine.anatomical_structure 030228 respiratory system biology.protein Neurology (clinical) medicine.symptom Muscle contraction |
| Zdroj: | Muscle & Nerve. 56:282-291 |
| ISSN: | 0148-639X |
| Popis: | Introduction Respiratory and locomotor skeletal muscle dysfunction are common findings in chronic obstructive pulmonary disease (COPD); however, the mechanisms that cause muscle impairment in COPD are unclear. Because Ca2+ signaling in excitation–contraction (E-C) coupling is important for muscle activity, we hypothesized that Ca2+ dysregulation could contribute to muscle dysfunction in COPD. Methods Intercostal and flexor digitorum brevis muscles from control and cigarette smoke-exposed mice were investigated. We used single cell Ca2+ imaging and Western blot assays to assess Ca2+ signals and E-C coupling proteins. Results We found impaired Ca2+ signals in muscle fibers from both muscle types, without significant changes in releasable Ca2+ or in the expression levels of E-C coupling proteins. Conclusions Ca2+ dysregulation may contribute or accompany respiratory and locomotor muscle dysfunction in COPD. These findings are of significance to the understanding of the pathophysiological course of COPD in respiratory and locomotor muscles. Muscle Nerve 56: 282–291, 2017 |
| Databáze: | OpenAIRE |
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