Polymorphism ofLRP5, but not ofTNFRSF11B, is associated with a decrease in bone mineral density in postmenopausal maya-mestizo women
| Autor: | Thelma Canto-Cetina, Ramón Mauricio Coral-Vázquez, Lizbeth González Herrera, David Rojano-Mejía, Agustín Coronel, Patricia Canto, Lucila Polanco Reyes |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Bone mineral
education.field_of_study medicine.medical_specialty Population Osteoporosis Haplotype Single-nucleotide polymorphism Biology medicine.disease medicine.anatomical_structure Endocrinology Anthropology Internal medicine Genotype Genetics medicine Anatomy education Genotyping Ecology Evolution Behavior and Systematics Femoral neck |
| Zdroj: | American Journal of Human Biology. 25:713-718 |
| ISSN: | 1042-0533 |
| DOI: | 10.1002/ajhb.22464 |
| Popis: | Objective Osteoporosis is a complex disease characterized principally by low bone mineral density (BMD), which is determined by an interaction of genetic, metabolic, and environmental factors. The aim of this study was to analyze the possible association among one polymorphism of LRP5 and three polymorphisms of TNFRSF11B as well as their haplotypes with BMD variations in Maya-Mestizo postmenopausal women. Methods We studied 583 postmenopausal women of Maya-Mestizo ethnic origin. A structured questionnaire for risk factors was applied and BMD was measured in lumbar spine (LS), total hip (TH), and femoral neck (FN) by dual-energy X-ray absorptiometry. DNA was obtained from blood leukocytes. One single-nucleotide polymorphism of LRP5 (rs3736228, p.A1330V) and three of TNFRSF11B (rs4355801, rs2073618, and rs6993813) were studied using real-time PCR allelic discrimination for genotyping. Differences between the means of the BMDs according to the genotype were analyzed with covariance. Deviations from Hardy–Weinberg equilibrium were tested. Pairwise linkage disequilibrium between single nucleotide polymorphisms was calculated by direct correlation r2, and haplotype analysis of TNFRSF11B was conducted. Results The Val genotype of the rs3736228 (p.A1330V) of LRP5 was significantly associated with BMD variations at the LS, TH, and FN. None of the three polymorphisms of TNFRSF11B was associated with BMD variations. Conclusions Our results show that p.A1330V was significantly associated with BMD variations at all three skeletal sites analyzed; the Val allele and the Val/Val genotype were those most frequently found in our population. Am. J. Hum. Biol. 25:713–718, 2013. © 2013 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text