Toluene exposure enhances acute and chronic formalin-induced nociception in rats: Participation of 5-HT 3 receptors
| Autor: | Daniel Godínez-Hernández, Marcia Yvette Gauthereau-Torres, Claudia Cervantes-Durán, Luis Fernando Ortega-Varela, Vinicio Granados-Soto |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Agonist medicine.drug_class Chemistry General Neuroscience Pharmacology Toxicology Receptor antagonist 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Nociception Allodynia Alosetron Anesthesia Hyperalgesia medicine medicine.symptom Receptor 030217 neurology & neurosurgery 5-HT receptor medicine.drug |
| Zdroj: | NeuroToxicology. 63:97-105 |
| ISSN: | 0161-813X |
| DOI: | 10.1016/j.neuro.2017.09.010 |
| Popis: | The purpose of this study was to evaluate the effect of acute toluene exposure on formalin (0.5% and 1%)-induced acute and long-lasting nociceptive hypersensitivity in rats. In addition, we sought to investigate the role of peripheral 5-HT3 receptors in the pronociceptive effect of toluene. Toluene exposure (6000ppm) for 30min enhanced 0.5% or 1% formalin-induced acute nociception and long-lasting secondary allodynia and hyperalgesia. In contrast, exposition to toluene for 30min in rats previously injected (six days before) with 1% formalin did not affect long-lasting hypersensitivy. Local peripheral pre-treatment with alosetron (5-HT3 receptor antagonist, 10-100 nmol) reduced the pronociceptive effect of toluene in acute nociception and long-lasting secondary allodynia and hyperalgesia. Alosetron (100nmol) was also able to reduce the nociceptive effects of 1% formalin in absence of toluene. Moreover, local peripheral injection of m-CPBG (5-HT3 receptor agonist, 300 nmol) enhanced 0.5% formalin-induced acute and long-lasting nociception in air- and toluene-exposed rats. Alosetron (10nmol) blocked the pronociceptive effects of m-CPBG (300nmol) on 0.5% formalin-induced acute and long-lasting hypersensitivity in rats exposed to toluene. Alosetron (at 10nmol) did not modify formalin-induced nociceptive behaviors. Finally, local peripheral pre-treatment with methiothepin (non-selective 5-HT receptor antagonist, 1.5nmol), did not affect the pronociceptive effect of toluene on 1% formalin-induced acute and long-lasting hypersensitivity. Our data demonstrate that acute exposure to toluene has pronociceptive effects in formalin-induced acute nociception and long-lasting hypersensitivity. Our data suggest that this pronociceptive effect depend on activation of peripheral 5-HT3, but not methiothepin-sensitive 5-HT, receptors. |
| Databáze: | OpenAIRE |
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