Modulation and Recruitment of Inducible Regulatory T Cells by First Trimester Trophoblast Cells
| Autor: | Ingrid Cardenas, Ayesha B. Alvero, Gil Mor, Laura Fraccaroli, Rosanna Ramhorst, Paulomi Aldo, Claudia Perez Leiros |
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| Rok vydání: | 2011 |
| Předmět: |
Interleukin 2
education.field_of_study Cellular differentiation medicine.medical_treatment Immunology Population Obstetrics and Gynecology Trophoblast Transforming growth factor beta Biology Immune tolerance Cell biology medicine.anatomical_structure Cytokine Reproductive Medicine Cell culture medicine biology.protein Immunology and Allergy education medicine.drug |
| Zdroj: | American Journal of Reproductive Immunology. 67:17-27 |
| ISSN: | 1046-7408 |
| DOI: | 10.1111/j.1600-0897.2011.01056.x |
| Popis: | Citation Ramhorst R, Fraccaroli L, Aldo P, Alvero AB, Cardenas I, Leiros CP, Mor G. Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cells. Am J Reprod Immunol 2012; 67: 17–27 Problem The specialized regulatory T-cells (Treg) population, essential for maternal tolerance of the fetus, performs its suppressive actions in the critical peri-implantation phase of pregnancy. In the present work, we investigated whether trophoblast cells are able to induce Treg recruitment, differentiation, and whether these mechanisms are modified by a bacterial or viral infection. Method of Study Human T-regulatory cells were differentiated from naive CD45RA+ CCR7+ cells obtained from peripheral blood mononuclear cells cultured with IL-2 and TGFβ over 5 days. Induction of iTregs (CD4+ Foxp3+ cells) was evaluated using low serum conditioned media (LSCM), obtained from two first trimester trophoblast cell lines, Swan-71 and HTR8. Coculture experiments were carried out using transwell assays where trophoblast cells were in the absence or presence of PGN, LPS, or Poly [I:C]. Cytokine production was measured by multiplex analysis. Results Trophoblast cells constitutively secrete high levels of TGFβ and induced a significant increase of Foxp3 expression accompanied by a specific T-reg cytokine profile. Moreover, trophoblast cells were able to recruit iTregs in a specific manner. Conclusion We demonstrate that trophoblast cells have an active role on the recruitment and differentiation of iTregs, therefore, contributing to the process of immune regulation at the placental–maternal interface. |
| Databáze: | OpenAIRE |
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