| Autor: |
L. T. Iguma, J. N. S. Sales, R. I. T. P. Batista, M. M. Pereira, C. C. R. Quintão, R. S. Verneque, L. S. A. Camargo, J. H. M. Viana, P. S. Baruselli |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Reproduction, Fertility and Development. 23:213 |
| ISSN: |
1031-3613 |
| DOI: |
10.1071/rdv23n1ab229 |
| Popis: |
Recent data reveal that approximately 80% of bovine embryos produced in vitro worldwide are from (Brazil J. H. M. Viana, personal communication). Adoption of this biotechnology by Brazilian producers might be attributed to particularities of the Bos indicus subspecies. Zebu breeds provide 2 to 3 times more viable oocytes than Bos taurus breeds per ovum pickup (OPU) session. This work aimed to evaluate the quality of cumulus–oocyte complexes (COC) retrieved from Gir breed cows (B. indicus) by OPU with a synchronized follicular wave and subjected to an in vitro embryo production (IVP) technique. All COC were obtained by OPU of 14 Gir cows performed every 14 days. There were 4 OPU sessions, preceded by synchronization of the follicular waves. Wave synchronization was achieved by an injection of 2 mg of oestradiol benzoate followed by the insertion of a norgestomet implant that stayed in place for 5 days. On Day 5, follicles of 2 to 8 mm were aspirated. The oocytes recovered were distributed according to classification: Group 1, with ≥3 cumulus cell layers and homogeneous or slightly heterogeneous cytoplasm (n = 314); and Group 2, ≤2 cumulus cells layers and slightly or coarsely granulated cytoplasm (n = 236). Oocytes from both groups were followed to in vitro maturation–IVF–fertilization–in vitro culture. Two (Day 2) and 8 (Day 8) days after the beginning of fertilization, the cleavage and blastocyst rates were measured, respectively. The statistical analyses were performed using a chi-square test (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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