Preservation of honey bee spermatozoa using egg yolk and soybean lecithin-based semen extenders and a modified cryopreservation protocol
| Autor: | Brandon K. Hopkins, M Emamverdi, Gholamali Nehzati-Paghaleh, Mahdi Zhandi, Fatemeh Dadkhah |
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| Rok vydání: | 2016 |
| Předmět: |
030219 obstetrics & reproductive medicine
food.ingredient urogenital system Extender 0402 animal and dairy science Semen 04 agricultural and veterinary sciences Honey bee Biology 040201 dairy & animal science Cryopreservation law.invention 03 medical and health sciences Honey Bees 0302 clinical medicine food law Insect Science Yolk Botany Soybean Lecithin Food science Sperm motility |
| Zdroj: | Journal of Apicultural Research. 55:279-283 |
| ISSN: | 2078-6913 0021-8839 |
| Popis: | Better protocols and suitable extenders for semen handling and cryopreservation are required for honey bees to enhance sperm quality and fertility similar to that of other species. The purpose of the present research is the improvement of honey bee sperm quality using different semen extenders and a modified cryopreservation protocol. Three different extenders were used: (1) buffer and egg yolk (EY); (2) buffer and 0.5% soybean lecithin (SL0.5); and (3) buffer and 2% soybean lecithin (SL2). Semen was collected and then diluted with the experimental extenders. The diluted semen was gradually cooled in a refrigerator to 5 °C and immediately loaded into straws and frozen with liquid nitrogen. Motility and viability data analyzed using the GENMOD and GLM procedure of SAS software. The results demonstrate that the mean fresh sperm motility in EY (4.75 ± 0.14) and SL2 (4.5 ± 0.2) were significantly (p |
| Databáze: | OpenAIRE |
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