| Popis: |
In this work, a comparative structural in silico analysis of two related ColE1-type plasmids, pCS36-4CPA and pRHBSTW-00138_7, was provided. At the nucleotide sequence level, their identity was about 82.2%. In the plasmid pRHBSTW-00138_7, modules of replication, propagation, stability, as well as a cargo module were identified. The plasmid was identified as a member of the ColE1 family. The stability module was provided by the plasmid DNA multimer resolution site (mrs). At the nucleotide level, the mrs of both plasmids had a 61.3% identity. In the structure of mrs pRHBSTW-00138_7, despite the high identity of pCS36-4CPA, significant structural modifications were found. Among them, the change in the secondary structure of the RNA transcript Rcd. The results obtained indicate that, although the mrs of both plasmids have a fundamentally the same structure, they probably could have been obtained by them independently of different ancestral plasmids. |
