| Popis: |
This study was conducted to optimize the supercritical carbon dioxide (SC-CO 2 ) extraction of Piper nigrum L. essential oil using response surface methodology (RSM). In order to obtain the maximum yield of the essential oil, experiments were carried out using a three-factor central composite design (CCD) under following conditions: pressure of 15–30 MPa, temperature of 40–50 °C and dynamic extraction time of 40–80 min. A second-order polynomial regression model expressing the total extraction yield as a function of main SC-CO 2 variables was significantly ( p R 2 > 0.985). The results showed that the best extraction yield (2.16%) was obtained at 30 MPa, 50 °C and 80 min. Pressure showed the most significant ( p 3.0%, calculated as % peak area) in the P. nigrum L. essential oil obtained through SC-CO 2 extraction were determined to be β -caryophyllene (24.34%), limonene (15.84%), sabinene (15.04%), 3-carene (9.44%), β -pinene (9.27%) and copaene (4.52%). |
Full Text from ScienceDirect