| Popis: |
Publisher Summary This chapter discusses the chromatographic analysis of pteridines. Pteridines are present in almost all types of biological tissues. Tetrahydrobiopterin is of interest to many investigators because of its probable role as a cofactor in vivo in mediating several mixed-function oxygenases. Much of the work in the studies of biopterin has been directed toward developing the methods for the accurate measurement of biopterin levels in various tissues, and fluids of man and other mammals. The lack of direct assays of adequate sensitivity has limited the quantitation of pteridines in studies on tissue distribution and metabolism. The separation of several pteridines by high-performance liquid chromatography (HPLC) has been reported, using columns, packed with a cation-exchange material. A reverse-phase column has been used for HPLC separations of folates and several 2,4-diaminopteridines. In this chapter, three different types of sephadex columns are evaluated for the separation of various pteridines. A method for the separation and quantitation of several pteridines by reverse-phase HPLC is described in this chapter. The chapter also discusses the microanalytical procedure for the extraction and analysis of biopterin from tissues and fluids, using HPLC. |
