Comparison of molecularly imprinted plasmonic nanosensor performances for bacteriophage detection
| Autor: | Nilüfer Cihangir, Yeşeren Saylan, Özgecan Erdem, Adil Denizli |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Detection limit 010401 analytical chemistry Nanoparticle Nanotechnology Context (language use) 02 engineering and technology General Chemistry Polymer 021001 nanoscience & nanotechnology 01 natural sciences Catalysis 0104 chemical sciences chemistry Tap water Nanosensor Materials Chemistry Surface plasmon resonance 0210 nano-technology Plasmon |
| Zdroj: | New Journal of Chemistry. 44:17654-17663 |
| ISSN: | 1369-9261 1144-0546 |
| DOI: | 10.1039/d0nj04053c |
| Popis: | Contamination of drinking and surface waters with microorganisms is a problem that attracts attention because it threatens human health. One of the microorganisms that determine fecal contamination in water is a bacteriophage that infects coliform bacteria. A fast, real-time, sensitive, and low-cost alternative method is the nanosensor system for microorganism detection. This study aimed to develop a surface plasmon resonance nanosensor for the detection of T4 bacteriophages using nanoparticle- and nanofilm-based polymers. In this context, nanoparticle and nanofilm-based imprinted polymers were synthesized to develop nanosensors, and their efficacy was compared in fecal pollution determination in water. Following the characterization studies, nanoparticle and nanofilm-based plasmonic nanosensors were used to carry out kinetic studies in the 1 × 104–4 × 106 pfu mL−1 concentration range in buffer, sea, and tap water sample solutions. Reusability and selectivity studies were also performed. According to the results, the nanoparticle and nanofilm-based plasmonic nanosensors could measure with 98% and 81% precision, respectively. The limit of detection values was calculated as 6 × 103 pfu mL−1 and 8 × 103 pfu mL−1 for the nanoparticle and the nanofilm-based plasmonic nanosensors, respectively. In addition, the recovery value of the nanoparticle-based plasmonic nanosensor was calculated as 91–96%, while the nanofilm-based plasmonic nanosensor was calculated as 85–90% in tap and seawater samples. All results showed that both plasmonic nanosensors could detect with high selectivity, but the nanoparticle-based plasmonic nanosensor has higher sensitivity than the nanofilm-based plasmonic nanosensor. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|