Analysis of human fibroblasts in vitro—imaging conditions and cytochalasin treatment
| Autor: | Gregory S. Watson, Sverre Myhra, Christopher T. Gibson, Colm Cahill, Gillian Robin Bushell, F.M. Clarke |
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| Rok vydání: | 1999 |
| Předmět: |
Chemistry
General Physics and Astronomy Substrate (chemistry) Nanotechnology Surfaces and Interfaces General Chemistry Adhesion Condensed Matter Physics In vitro Surfaces Coatings and Films Tissue culture chemistry.chemical_compound medicine.anatomical_structure Cell culture Biophysics medicine Cytochalasin Cytoskeleton Fibroblast |
| Zdroj: | Applied Surface Science. :141-145 |
| ISSN: | 0169-4332 |
| Popis: | The structure of human fibroblasts has been characterised in vitro by atomic force microscopy (AFM) operated in the imaging or in the force vs. distance ( F – d ) modes. The choice of growth substrate is important to ensure good adhesion. The substrate also affects the imaging conditions for in vitro analysis of live cells; activated tissue culture dishes are shown to promote conditions that routinely result in good quality images. A qualitative model suggests that the activated substrate may act as a preferential scavenger of cellular debris, therefore promoting low adhesion between tip and membrane and preventing the tip from biofouling. Alternatively, the activated substrate may promote a more rigid cell structure, thus resulting in improved imaging. Good imaging conditions provide nondestructive in vitro information about cytoskeletal structure and dynamics; thus, treatment with cytochalasin can be monitored in real time for durations of several hours. |
| Databáze: | OpenAIRE |
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