Isolation of nucleated cells fraction from umbilical cord blood – choice of method
| Autor: | A. B. Smolyaninov, D. A. Ivolgin |
|---|---|
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Cell and Organ Transplantology. 2:30-33 |
| ISSN: | 2311-021X 2308-3794 |
| Popis: | Umbilical cord blood (CB) is now recognized as one of the main sources of stem cells for the treatment of various diseases. Apart from the advantages as compared to other sources of stem cells (bone marrow, peripheral blood), CB has a relatively limited number of hematopoietic cells [1, 2]. Since the outcome of transplantation depends on the number of transplanted cells [3], there is a need to minimize the loss of cells during sample processing. Studies held in CB banks to increase the efficiency of total nucleated cells (TNCs) allocating can significantly improve the outcomes of stem CB cell transplantation [4, 5]. The idea of processing (volume reduction) of CB placed for a longterm cryostorage, belongs to the founder of the first public cord blood bank Professor P. Rubinstein (1995). Intact CB was placed for storage before. Processing of CB pursued the reduction of storage space and related costs; and the reduction of cryoprotectant addition into CB sample to lower its side effects in transplantation. The first approach to reduce the CB sample volume became the double centrifugation method [6]. This method and its modifications [7, 8] are used to date. They are a standard for comparing the efficiency of TNCS fraction allocation with other techniques. The next step in improving of CB processing technology has been the emergence of so-called semi-automatic systems «top-and-bottom», originally used for the fractionation of peripheral blood [9]. The examples of such systems are «Optipress II» (Baxter, USA) and Compomat G4 (Fresenius, Germany). To date, these systems are widely used to extract a fraction of TNCs out of CB [10-13]. Their effectiveness was evaluated in the framework of technological solutions of semi-automatic separation systems [14], and in comparison with other CB processing methods [15, 16]. Experience shows clear perspectives of semi-automatic systems [17] to satisfy high and stable CB cells yield. Furthermore, the use of semi-automatic CB processing systems significantly reduces the risk of contamination and damage of CB container [18]. Since 2000 along with the use of semi-automatic systems there were used fully automatic ones, such as Sepax S-100 (Biosafe, Switzerland) [19, 20]. Using this cell separator, a number of manipulations with CB is reduced comparing to the semi-automatic systems. In addition, such an apparatus has programs allowing the selection of the cell concentrate from small CB sample and washing off the cryoprotectant from thawed cell concentrate. There are other CB processing methods, but they are not widely available at the moment [21]. Nevertheless, in a CB bank, treatment and storage organization of CB samples should consider not only the effectiveness of various methods to extract a fraction of TNCs out of CB, but also costs of equipment and supplies, and processing time. ABSTRACT |
| Databáze: | OpenAIRE |
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