Effects of Cu 2+ and pH on osteoclastic bone resorption in vitro *

Autor:	Jian Huang, Jinchao Zhang, Kui Wang, Shanjin Xu, Shifeng Yu
Rok vydání:	2003
Předmět:	inorganic chemicals musculoskeletal diseases chemistry Scanning electron microscope Mole Biophysics chemistry.chemical_element General Materials Science Japanese white rabbit Anatomy Calcium Bone resorption In vitro
Zdroj:	Progress in Natural Science. 13:266-270
ISSN:	1745-5391 1002-0071
DOI:	10.1080/10020070312331343510
Popis:	The effects of Cu2+ and pH on osteoclastic bone resorption lacunae are studied by culturing Japanese white rabbit osteoclasts on bone slices. The number and surface area of bone resorption lacunae are measured by photomicrography and image analysis. Concentration of calcium ion in the supernatant is measured by atomic absorption spectrometry to evaluate the activity of osteoclasts. The morphology of the lacunae is observed under a scanning electron microscope. The results indicate that Cu2+ at concentration of 1.00 × 10−6 mol/L and 1. 00 × 10−7 mol/L inhibits osteoclastic activity and causes a dose-dependent reduction in the number and surface area of the lacunae. While the number of lanunae is increased and osteoclastic bone resorbing function is significantly improved at Cu2+ concentration of 1.00 × 10−8 mol/L. It is suggested that the effect of Cu2+ on osteoclastic bone resorption depends on Cu2+ concentration. pH had no significant effect on osteoclastic function in the near neutral range.
Databáze:	OpenAIRE
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