Determination of Individual Carotenoids in Human Plasma by High Performance Liquid Chromatography

Autor:	John G. Bieri, J. Cecil Smith, Ellen D. Brown
Rok vydání:	1985
Předmět:	chemistry.chemical_classification Chromatography Chemistry food and beverages Chloride High-performance liquid chromatography Solvent chemistry.chemical_compound Echinenone medicine Molecular Medicine Methanol Methylene Acetonitrile Carotenoid medicine.drug
Zdroj:	Journal of Liquid Chromatography. 8:473-484
ISSN:	0148-3919
DOI:	10.1080/01483918508067094
Popis:	A high performance liquid chromatography procedure using an internal carotenoid standard, echinenone, is described. The method uses a C-18, reverse-phase column, an isocratic solvent (acetonitrile:methylene chloride:methanol, 70:20:10) and requires only thirteen minutes. Most human plasmas gave seven distinct peaks; six carotenoids are identified. The sum of seven peaks is 90 percent or more of the total carotenoids in a total lipid extract.
Databáze:	OpenAIRE
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