TLR4 in monocytes plays a pivotal role on monocyte adhesion to vascular endothelium (LB226)

Autor: Jin-Ung Bae, Kyo-Won Seo, Seung-Jin Lee, Chi Dae Kim
Rok vydání: 2014
Předmět:
Zdroj: The FASEB Journal. 28
ISSN: 1530-6860
0892-6638
Popis: Toll-like receptor 4 (TLR4) is known to mediate monocyte adhesion to endothelial cells via an increased expression of adhesion molecules on endothelial cells (EC), however, its role on the expression of monocyte adhesion molecules is unclear. In the present study, we investigated the role of TLR4 on the expression of monocyte adhesion molecules, and determined the functional role of TLR4-induced adhesion molecules on monocyte adhesion to vascular endothelium. When THP-1 monocytes were stimulated with Kdo2-Lipid A (KLA), a specific TLR4 agonist, Mac-1 expression was markedly increased in association with an increased adhesion of monocytes to EC. These were attenuated by anti-Mac-1 antibody, suggesting a functional role of TLR4-induced Mac-1 on monocyte adhesion on EC. In monocytes treated with MK886, a 5-lipoxygenase (LO) inhibitor, both Mac-1 expression and monocyte adhesion to EC induced by KLA were markedly attenuated. Moreover, KLA increased the expression of mRNA and protein of 5-LO, suggesting a pivo...
Databáze: OpenAIRE
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