Determination of Arteether in Plasma Using a Simple and Rapid High-Performance Liquid Chromatographic Assay
| Autor: | A. A. Al-Angary, M. A. Al-Meshal, Mohsen A. Bayomi, S. H. Khidr, K. M. Lutfi |
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| Rok vydání: | 1994 |
| Předmět: |
Detection limit
Chromatography Biochemistry (medical) Clinical Biochemistry Derivative Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound chemistry Electrochemistry Protein precipitation Acid hydrolysis Perchloric acid Acetonitrile Quantitative analysis (chemistry) Spectroscopy |
| Zdroj: | Analytical Letters. 27:2689-2702 |
| ISSN: | 1532-236X 0003-2719 |
| DOI: | 10.1080/00032719408006000 |
| Popis: | A simple and rapid high-performance liquid chromatographic (HPLC) assay for the determination of the antimalarial drug arteether in plasma was developed and validated in this report. Perchloric acid was used in this method as a plasma protein precipitant and to attain an acidic medium suitable for the decomposition of arteether to a derivative possessing UV absorption. This derivative and the internal standard (progesterone) were separated from the plasma on a 10 μm μ-Bondapack C18 reversed-phase column at ambient temperature with a mobile phase composed of acetonitrile:water (60:40 v/v) and at a flow rate of 1.5 ml/min. The effluent was monitored at 254 nm with a UV detector. Linear relation between drug concentrations and peak height ratios of arteether derivative to the internal standard was achieved in the range of 0.25-10 μg/ml arteether with a detection limit of 50 ng/ml arteether in plasma. The within-day and between-days precisions were evaluated using 3 different concentrations of arteet... |
| Databáze: | OpenAIRE |
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