Comprehensive genomics depict accessory genes encoding pathogenicity and biofilm determinants in Enterococcus faecalis
| Autor: | Karthika Suryaletha, Sabu Thomas, Sivakumar Krishnankutty Chandrika |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Comparative genomics biology 030106 microbiology Biofilm Pan-genome Virulence biochemical phenomena metabolism and nutrition biology.organism_classification Microbiology Pathogenicity island Enterococcus faecalis 03 medical and health sciences 030104 developmental biology Plasmid Pathogen |
| Zdroj: | Future Microbiology. 16:143-157 |
| ISSN: | 1746-0921 1746-0913 |
| DOI: | 10.2217/fmb-2020-0111 |
| Popis: | Aim: Enterococcus faecalis is a leading nosocomial pathogen in biofilm-associated polymicrobial infections. The study aims to understand pathogenicity and biofilm determinants of the pathogen by genome analysis. Methodology: Genome sequencing of a strong biofilm forming clinical isolate Enterococcus faecalis SK460 devoid of Fsr quorum-signaling system, was performed and comparative genomics was carried out among a set of pathogenic biofilm formers and nonpathogenic weak biofilm formers. Results: Analysis revealed a pool of virulence and adhesion related factors associated with pathogenicity. Absence of CRISPR-Cas system facilitated acquisition of pheromone responsive plasmid, pathogenicity island and phages. Comprehensive analysis identified a subset of accessory genes encoding polysaccharide lyase, sugar phosphotransferase system, phage proteins and transcriptional regulators exclusively in pathogenic biofilm formers. Conclusion: The study identified a set of genes specific to pathogenic biofilm formers and these can act as targets which in turn help to develop future treatment endeavors against enterococcal infections. |
| Databáze: | OpenAIRE |
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