Current molecular profile of juvenile nasopharyngeal angiofibroma: First comprehensive study from India
| Autor: | Shivani Pandey, Vinay Singh, Hitendra Prakash Singh, Amita Pandey, Ashoak Mani Tripathi, Praveen Pandey, Veerendra Verma, Brijesh Patel, Subhash C. Mishra, Anupam Mishra, Ritu Trivedi, Sunil Kumar |
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| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
Platelet-derived growth factor biology Tumor suppressor gene Juvenile nasopharyngeal angiofibroma Basic fibroblast growth factor Nasopharyngeal neoplasm Androgen receptor 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Real-time polymerase chain reaction Otorhinolaryngology chemistry 030220 oncology & carcinogenesis Internal medicine medicine biology.protein Cancer research 030223 otorhinolaryngology Platelet-derived growth factor receptor |
| Zdroj: | The Laryngoscope. 127:E100-E106 |
| ISSN: | 0023-852X |
| Popis: | Objective An attempt is made to analyze the molecular behavior of juvenile nasopharyngeal angiofibroma (JNA). Study Design Case Series Methods Quantification of mRNAs expression was undertaken through real-time polymerase chain reaction in JNA (9–24) samples for VEGF-A, basic fibroblast growth factor (b-FGF), platelet-derived growth factor PDGF-A, KIT proto-oncogene receptor tyrosine kinase (c-Kit), Avian myelomatosis viral oncogene homolog (c-Myc), Harvey rat sarcoma viral oncogene homolog (H-Ras), tumor suppressor gene TP53, and androgen receptor and interleukin 6 (IL-6). The β-catenin expression was evaluated by western blot in 16 samples. Nasal polyp was taken as control. Results A significantly increased (P < 0.01) expression of c-myc, VEGFA, bFGF, PDGFA, c-kit, and TP53 was seen, along with enhanced expression of β-catenin. A massive enhancement of H-Ras expression was seen for the first time. Androgen receptor expression was no different, whereas IL-6 despite showing upregulation trend was not significant. Conclusion The enhanced expressions of various markers suggest their potential role in JNA. Although the biological significance of c-kit, c-myc, and one of the novel markers H-Ras has yet to be defined, their significant expression may have a therapeutic importance. Level of Evidence NA. Laryngoscope, 2016 |
| Databáze: | OpenAIRE |
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