EVALUATION OF DevR ANTIGEN (MSMEG_5244) AS A PUTATIVE BIOMARKER FOR THE SERODIAGNOSIS OF PERSISTANT Mycobacterium tuberculosis
| Autor: | S. Sambantham, S. Radhamahendran, R. Malathi, G. Jayaraman |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | International Journal of Molecular Biology. 4:81-86 |
| ISSN: | 0976-0490 0976-0482 |
| Popis: | Tuberculosis (TB) is a major health problem in the developing world. 9 million new cases of TB and three million deaths are report- ed every year around the globe. The management of tuberculosis in the regions of developing countries urgently needs an efficient diagnostic test. DevR (also called DosR) is essential for promoting long-term survival of Mycobacterium tuberculosis under low oxygen conditions in a dormant state and may be responsible for latent tuberculosis in one-third of the world's population. The similarity in sequences of DevR in Mycobacterium smegmatis and Mycobacterium tuberculosis has been already proved and hence My- cobacterium smegmatis is used as a model in present study for studying and evaluating the immunogenic properties of antigen DevR. Elimi- nation of tuberculosis (TB) largely depends upon definitive rapid diagnosis and treatment. Widely used diagnostic methods do not qualify for use in a developing country due to lack of either desired accuracy and cost. In the present study ELISA was used to evaluate the diagnostic potential of an immuno-dominant antigen DevR from 36 BCG vaccinated and 12 non-vaccinated TB patients. 48 samples were collected from healthy control subjects. We have evaluated the diagnosis of tuberculosis, based on detection of anti-IgG antibodies in the TB patient's sera. The mean IgG antibody levels in sera of both BCG vaccinated (n=36) and non-vaccinated (n=12) patients with tuberculosis were significantly higher compared to the healthy donors (p |
| Databáze: | OpenAIRE |
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