Deacetylcephalosporin C formation by cephalosporin C acetylhydrolase induced in a Cephalosporium acermonium mutant
| Autor: | Toshihiko Kanzaki, Hideo Shirafuji, Yukio Fujisawa |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
inorganic chemicals
chemistry.chemical_classification Chromatography biology Stereochemistry Deacetylcephalosporin C Mutant Cephalosporin C General Biochemistry Genetics and Molecular Biology Enzyme assay chemistry.chemical_compound Column chromatography Enzyme chemistry Cephalosporium acremonium biology.protein Cephalosporin C acetylhydrolase General Agricultural and Biological Sciences |
| Zdroj: | Agricultural and Biological Chemistry. 39:1303-1309 |
| ISSN: | 1881-1280 0002-1369 |
| DOI: | 10.1271/bbb1961.39.1303 |
| Popis: | Cephalosporin C acetyl-hydrolase, which had not yet been found in Cephalosporium acremonium cultures, was partially purified from the culture fluid of the mutant No. 81 by ammonium sulfate fractionation, dialysis and DEAE-cellulose column chromatography. The optimum pH and temperature of the enzyme reaction were found to be about 8.0 and 50°C, respectively. The enzyme activity was hardly affected by Mg2+, Mn2+, Zn2+, Co2+, Ni2+, Na+, K+, EDTA, PCMB and 2,4-dinitrophenol, but markedly inhibited by diisopropylfluoro-phosphate at 1 mm. The product formed from cephalosporin C by the enzyme reaction was proved to be deacetylcephalosporin C by physical and chemical analyses and chromatographic behaviors. |
| Databáze: | OpenAIRE |
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