Effect of CWG methylation on expression of plant genes

Autor:	Nigel A. R. Urwin, Sriharsa Pradhan, Gareth I. Jenkins, Roger L. P. Adams
Rok vydání:	1999
Předmět:	Genetics Reporter gene Methyltransferase biology Promoter Cell Biology Methylation biology.organism_classification Biochemistry Molecular biology In vitro chemistry.chemical_compound chemistry Transcription (biology) Cauliflower mosaic virus Molecular Biology DNA
Zdroj:	Biochemical Journal. 341:473-476
ISSN:	1470-8728 0264-6021
DOI:	10.1042/bj3410473
Popis:	The presence of two DNA methyltransferases in Pisum raises the possibility that they serve different functions. In vitro methylation of CWG sequences in the strong cauliflower mosaic virus 35S promoter had no effect on reporter gene expression. In contrast, in vitro methylation of CWG sequences in the relatively weak, CG-deficientPhaseolus vulgaris rbcS2 promoter inhibited transcription. Expression of both constructs was strongly inhibited by extensive CG methylation. A search of published plant promoter sequences revealed that the CG content of promoters is very variable, with some promoters having typical CG islands. In contrast, the distribution of CWG sequences is more even with little evidence for CWG islands.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f2e7030c99842825759b89c0e498d49d https://doi.org/10.1042/bj3410473 Zobrazit plný text záznamu