Induction of DNA Damage in L5178Y Cells Treated with Gold Nanoparticle
| Autor: | Joo Hwan Kim, Bong Hyun Chung, Young Na Yum, Jinyoung Jeong, Jin Seok Kang, Yong Taik Lim, Sue Nie Park, Hyun A Song |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Pharmacology
Scanning electron microscope Chemistry DNA damage medicine.disease_cause Biochemistry Molecular biology Dark field microscopy Comet assay Reverse transcription polymerase chain reaction Transmission electron microscopy Drug Discovery medicine Molecular Medicine Cytotoxicity Oxidative stress |
| Zdroj: | Biomolecules and Therapeutics. 17:92-97 |
| ISSN: | 1976-9148 |
| Popis: | As nanomaterials might enter into cells and have high reactivity with intracellular structures, it is necessary to assay possible genotoxic risk of them. One of these approaches, we investigated possible genotoxic potential of gold nanoparticle (AuNP) using L5178Y cells. Four different sizes of AuNP (4, 15, 100 or 200 nm) were synthesized and the sizes and structures of AuNP were analyzed using transmission electron microscopy (TEM), scanning electron microscopy (SEM) and stability was analyzed by a UV/Vis. Spectrophotometer. Cytotoxicity was assessed by direct cell counting, and cellular location was detected by dark field microscope at 6, 24 and 48 h after treatment of AuNP. Comet assay was conducted to examine DNA damage and tumor necrosis factor (TNF)-α mRNA level was assay by real-time reverse transcription polymerase chain reaction. Synthetic AuNP (4, 50, 100 and 200 nm size) had constant characteristics and stability confirmed by TEM, SEM and spectrophotometer for 10 days, respectively. Dark field microscope revealed the location of AuNP in the cytoplasm at 6, 24 and 48 h. Treatment of 4 nm AuNP induced dose and time dependent cytotoxicity, while other sizes of AuNP did not. However, Comet assay represented that treatment of 100 nm and 200 nm AuNP significantly increased DNA damage compared to vehicle control (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|