Improved enzymatic performance of graphene-immobilized β-glucosidase A in the presence of glucose-6-phosphate
| Autor: | Luiz Claudio Miletti, Gustavo Felippe da Silva, Everton Skoronski, Anderson Albino Gomes, Elisa Pazinatto Telli, Maria de Lourdes Borba Magalhães, Marcos Gomes Ghislandi |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Immobilized enzyme Biomedical Engineering Biomass Lignocellulosic biomass Bioengineering 01 natural sciences Applied Microbiology and Biotechnology Catalysis 03 medical and health sciences chemistry.chemical_compound Hydrolysis 010608 biotechnology Enzymatic hydrolysis Drug Discovery Cellulose chemistry.chemical_classification Process Chemistry and Technology Biomolecule General Medicine Combinatorial chemistry 030104 developmental biology chemistry Biochemistry Molecular Medicine Biotechnology |
| Zdroj: | Biotechnology and Applied Biochemistry. 65:246-254 |
| ISSN: | 0885-4513 |
| DOI: | 10.1002/bab.1569 |
| Popis: | Optimization of cellulose enzymatic hydrolysis is crucial for cost-effective bioethanol production from lignocellulosic biomass. Enzyme immobilization in solid support allows enzyme recycling for reuse, lowering hydrolysis costs. Graphene is a nanomaterial isolated in 2004, which possesses exceptional properties for biomolecule immobilization. This study evaluates the potential for β-glucosidase recycling by immobilization on graphene nanosheets. Data reported here demonstrated that graphene-immobilized β-glucosidase can be recycled for at least eight cycles. Immobilization did not change the optimal temperature of catalysis and improved enzymatic stability upon storage. The role of glucose-6-phosphate on immobilized enzyme was also investigated, demonstrating that glucose-6-phosphate acts as a mixed-type activator and improves storage stability of immobilized enzyme. Complete cellulose hydrolysis using graphene-immobilized β-glucosidase in the presence of glucose-6-phosphate resulted in greatly improved hydrolysis rates, demonstrating the potential of this strategy for biomass hydrolysis. |
| Databáze: | OpenAIRE |
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