Optimal Voltage and Electrical Pulse Conditions for Electrical Ablation to Induce Immunogenic Cell Death (ICD)
| Autor: | Wooram Park, Dong Keun Han, Chan Kim, Kyung-Soon Park, Dong-Hyun Kim, Eun Jin Go, Hong Jae Chon, WonHyoung Ryu, Dasom Yang |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
Pulse (signal processing) General Chemical Engineering medicine.medical_treatment 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Ablation 01 natural sciences 0104 chemical sciences Cancer immunotherapy In vivo Cancer cell Cancer research medicine Immunogenic cell death Viability assay 0210 nano-technology Ablation zone |
| Zdroj: | Journal of Industrial and Engineering Chemistry. 94:225-232 |
| ISSN: | 1226-086X |
| Popis: | Electrical ablation (EA) is a non-thermal ablation technique that causes less damage to the tissue surrounding the targeted area than conventional thermal ablation. EA induces immunogenic cell death (ICD) and can potentially synergize with cancer immunotherapy. In this study, the optimal voltage and pulse conditions for the induction of ICD were identified. Finite element analysis was used to estimate the ablation zone under a variety of voltage and pulse conditions. Cancer cells were cultured in vitro under two- and three-dimensional conditions to assess cell viability under different voltage and pulse conditions. Additionally, the expression of damage-associated molecular pattern (DAMP) markers of ICD was measured to identify an optimal voltage condition. Tumor volume, body weight, and survival after EA treatment were measured in vivo to identify the optimal pulse conditions. The optimal conditions to induce ICD by EA identified in this study are suitable to be combined with cancer immunotherapy. |
| Databáze: | OpenAIRE |
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