MGE-derived nNOS + interneurons promote fear acquisition in nNOS −/− mice
Autor: | Bo Cao, Hong-Jin Yuan, Cheng-Cheng Kong, Yan Liu, Chun-Xia Luo, Jun Li, Fang Yuan, Lei Chang, Hai-Yin Wu, Lin Zhang, Huan-Yu Ni |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Ganglionic eminence Chemistry Dentate gyrus Central nervous system Biophysics Cell Biology Anatomy musculoskeletal system Biochemistry Embryonic stem cell Cortex (botany) Cell biology body regions Transplantation Preoptic area 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure nervous system cardiovascular system medicine Biological neural network Molecular Biology reproductive and urinary physiology |
Zdroj: | Biochemical and Biophysical Research Communications. 493:1560-1566 |
ISSN: | 0006-291X |
Popis: | Neuronal nitric oxide synthase (nNOS) 1 , mainly responsible for NO release in central nervous system (CNS) 2 , plays a significant role in multiple physiological functions. However, the function of nNOS + interneurons in fear learning has not been much explored. Here we focused on the medial ganglionic eminences (MGE) 3 -derived nNOS + interneurons in fear learning. To determine the origin of nNOS + interneurons, we cultured neurons in vitro from MGE, cortex, lateral ganglionic eminence (LGE) 4 , caudal ganglionic eminences (CGE) 5 and preoptic area (POA) 6 . The results showed that MGE contained the most abundant precursors of nNOS + interneurons. Moreover, donor cells from E12.5 embryos demonstrated the highest positive rate of nNOS + interneurons compared with other embryonic periods (E11.5, E12, E13, E13.5 and E14). Additionally, these cells from E12.5 embryos showed long axonal and abundant dendritic arbors after 10 days culture, indicating the capability to disperse and integrate in host neural circuits after transplantation. To investigate the role of MGE-derived nNOS + interneurons in fear learning, donor MGE cells were transplanted into dentate gyrus (DG) 7 of nNOS knock-out (nNOS −/− ) or wild-type mice. Results showed that the transplantation of MGE cells promoted the acquisition of nNOS −/− but not the wild-type mice, suggesting the importance of nNOS + neurons in fear acquisition. Moreover, we transplanted MGE cells from nNOS −/− mice or wild-type mice into DG of the nNOS −/- mice and found that only MGE cells from wild-type mice but not the nNOS −/− mice rescued the deficit in acquisition of the nNOS −/− mice, further confirming the positive role of nNOS + neurons in fear learning. |
Databáze: | OpenAIRE |
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