Technique to minimize phenolics in walnut in vitro culture initiation

Autor:	Nova Ahmed, H. Itoo, Hidayatullah Mir, M. A. Sheikh, Shabir H. Wani, J. I. Mir, Rizwan Haroon Rashid
Rok vydání:	2017
Předmět:	chemistry.chemical_compound Horticulture Tissue culture chemistry Inoculation Shoot Phenol Phenols Ascorbic acid Citric acid Explant culture
Zdroj:	Indian Journal of Horticulture. 74:285
ISSN:	0974-0112 0972-8538
DOI:	10.5958/0974-0112.2017.00057.3
Popis:	Total phenol content of the walnut genotypes was determined to explore the relationship between total phenols and regeneration response for different walnut genotypes. Walnut leaves from ten genotypes (CITHWalnut-I, CITH-Walnut-II, BBW-8, CITH-Walnut-IV, BP-3, SP-1, LG-11, Hamdan, Suleiman, and Opex Culchery) were used for extraction of total phenols through modified Folin-Ciocalteu method. Total leaf phenolic content ranges from 140 μg g−1 (WGB-1) to 285 μg g−1 (BBW-8). Phenolic interactions expressed as darkening of the explants lead to death. Among different antioxidants used ascorbic acid @ 350 mg/l was found best with almost no phenol exudation in the medium and shoot initiation occurred after 8 days of inoculation. The number of shoots was highest (10), followed by citric acid used @ 350 mg/l showing low degree of exudation where shoot initiation was noted in 15 days and with 15.0 shhots per explant.
Databáze:	OpenAIRE
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