Short Communication: Studies in Macaques on Cross-Clade T Cell Responses Elicited by a DNA/MVA AIDS Vaccine, Better Conservation of CD8 Than CD4 T Cell Responses
| Autor: | Harold M. McClure, Bernard Moss, Linda S. Wyatt, Harriet L. Robinson, Sunita Sharma, Lakshmi Chennareddi, Sal Butera, Janet M. McNicholl, James M. Smith, James G. Herndon, Dennis Ellenberger, Bin Li, Milloni Patel, Rama Rao Amara |
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| Rok vydání: | 2005 |
| Předmět: | |
| Zdroj: | AIDS Research and Human Retroviruses. 21:140-144 |
| ISSN: | 1931-8405 0889-2229 |
| DOI: | 10.1089/aid.2005.21.140 |
| Popis: | One of the unknowns faced by an HIV/AIDS vaccine is the ability of a single clade vaccine to protect against the multiple genetic subtypes and recombinant forms of HIV-1 present in the current pandemic. Here, we use a macaque model to investigate the ability of our clade B vaccine that consists of DNA priming and modified vaccinia Ankara (MVA) virus boosting to elicit T cell responses that recognize an A/G recombinant of HIV- 1. To test for cross-reactive T cells, intracellular cytokine staining was conducted using five pools of Gag and six pools of Env peptides representing B or A/G sequences. Studies using the peptide pools revealed essentially complete conservation of the CD8 response but only ∼50% conservation of the CD4 response. Thus, the ability of an HIV vaccine for one clade to protect against other clades may be more limited by the ability to provide CD4 T cell help than the ability to elicit CD8 effector functions. |
| Databáze: | OpenAIRE |
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