Secretion of Active Human Tissue Plasminogen Activator from the Filamentous Fungus Aspergillus Nidulans
| Autor: | G. L. McKnight, K. P. Lewison, M. C. Bailey, M. L. Joseph, J. M. Maraganore, A. Upshall, M. A. Favreau, M. D. Parker, A. A. Kumar |
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| Rok vydání: | 1987 |
| Předmět: |
biology
Biomedical Engineering Protein primary structure Bioengineering Biological activity biology.organism_classification Applied Microbiology and Biotechnology Tissue plasminogen activator Microbiology Aspergillus nidulans Polyclonal antibodies Complementary DNA medicine biology.protein Molecular Medicine Secretion Plasminogen activator Biotechnology medicine.drug |
| Zdroj: | Nature Biotechnology. 5:1301-1304 |
| ISSN: | 1546-1696 1087-0156 |
| DOI: | 10.1038/nbt1287-1301 |
| Popis: | Strains of the filamentous fungus Aspergillus nidulans transformed with a functional cDNA comprising the entire coding region of human tissue plasminogen activator (t-PA) secrete biologically active protein into the culture medium. Activity is inhibited by treatment with t-PA specific polyclonal antibodies. The protein is correctly processed at the amino terminus and is not excessively glycosylated. |
| Databáze: | OpenAIRE |
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