Isozymes as markers for identification of tissue culture and greenhouse-grown strawberry cultivars

Autor:	C. Stushnoff, N.S. Nehra, K.K. Kartha
Rok vydání:	1991
Předmět:	fungi food and beverages Dehydrogenase Plant Science Horticulture Biology Isozyme Aminopeptidase Esterase Tissue culture Biochemistry Phosphoglucomutase Agronomy and Crop Science Polyacrylamide gel electrophoresis Homogenization (biology)
Zdroj:	Canadian Journal of Plant Science. 71:1195-1201
ISSN:	1918-1833 0008-4220
DOI:	10.4141/cjps91-167
Popis:	Polyacrylamide gel electrophoresis (PAGE) was used for analysis of isozyme banding patterns of leucine aminopeptidase (LAP), phosphoglucomutase (PGM), phosphoglucoisomerase (PGI), esterase (EST) and 6-phosphogluconate dehydrogenase (6-PGD) in strawberry leaves. The extracts prepared from young leaf tissues using polytron homogenization and an extraction buffer containing 15 mg ml−1 dithiothreitol (DTT) and 10% insoluble polyvinylpyrrolidone (PVP-6755) gave best resolution for these enzymes. The influence of plant age and various growing environments on the stability of isozyme phenotypes was examined. The isozyme banding patterns of 6-PGD were found to vary with the change in growing environment as well as age of the plants. EST produced different banding patterns in greenhouse and tissue culture leaves. However, the isozyme phenotypes of LAP, PGM and PGI remained stable under all the conditions tested. Using a combination of these three stable enzymes, it was possible to distinguish eight strawberry cultivars under both tissue culture and greenhouse conditions. Key words: Fragaria × ananassa Duch., meristem culture, polyacrylamide gel electrophoresis
Databáze:	OpenAIRE
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