Sequence polymorphism in spacer ITS2 of Anopheles (Cellia) sub-pictus Grassi (Diptera: Culicidae)
| Autor: | Taruna Kaura, Monika Sharma, Asha Chaudhry, Sudarshan Chaudhry |
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| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Caryologia. 63:124-133 |
| ISSN: | 2165-5391 0008-7114 |
| DOI: | 10.1080/00087114.2010.10589717 |
| Popis: | Mosquito cytogenetic studies have revealed that the majority of disease vector species exist as cryptic species complexes. In relevance to this, the present results include the sequence analysis of r-DNA internal transcribed spacer 2 (ITS2) of two populations of Anopheles subpictus Grassi collected from Chandigarh (Pop.A) and Hoshiarpur, Punjab, India (Pop.B). These sequences were compared with five other populations C, D, E, F and G worked out earlier. ITS2 sequence in Pop. A was 681 bp compared to 491 bp in Pop. B. All the sequences were G:C rich. In populations A-E, which form the Indian component of An. subpictus, the ts/tv frequencies ranged from 0.25 to 0.77. Populations F and G, which represent the Srilankan fauna, were much higher at 0.83 to 0.90 in inland and coastal populations respectively. The SRF revealed various common interspersed repeats in the form of dimers, trimers, tetramers, pentamers and polymers. The TG repeat motif, which was repeated 50 to 60 times, was common in Indian a... |
| Databáze: | OpenAIRE |
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