Autor: |
Nadia López, María Eugenia Mejía, María Inés Baquero, Gabriel Trueba |
Rok vydání: |
2010 |
Předmět: |
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Zdroj: |
The Open Veterinary Science Journal. 4:31-35 |
ISSN: |
1874-3188 |
DOI: |
10.2174/1874318801004010031 |
Popis: |
Bovine leptospirosis is a highly prevalent infection worldwide causing serious losses in cattle production and serving as a source for human infection. Diagnosis and assessment of prevalence of this infection in bovine herds is difficult due to limitations of current procedures. The present report describes the adaptation of a polymerase chain reaction (PCR) protocol for detection of leptospiral DNA in bovine urine. The amplification products corresponded to a segment of the Leptospira 16S rRNA gene detected using two sets of primers (A/B and C/D). |
Databáze: |
OpenAIRE |
Externí odkaz: |
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