Evaluation of a Polymerase Chain Reaction for the Diagnosis of Leptospirosis in Cattle~!2010-03-10~!2010-08-06~!2010-09-06~!

Autor: Nadia López, María Eugenia Mejía, María Inés Baquero, Gabriel Trueba
Rok vydání: 2010
Předmět:
Zdroj: The Open Veterinary Science Journal. 4:31-35
ISSN: 1874-3188
DOI: 10.2174/1874318801004010031
Popis: Bovine leptospirosis is a highly prevalent infection worldwide causing serious losses in cattle production and serving as a source for human infection. Diagnosis and assessment of prevalence of this infection in bovine herds is difficult due to limitations of current procedures. The present report describes the adaptation of a polymerase chain reaction (PCR) protocol for detection of leptospiral DNA in bovine urine. The amplification products corresponded to a segment of the Leptospira 16S rRNA gene detected using two sets of primers (A/B and C/D).
Databáze: OpenAIRE