Development of conventional multiplex PCR method for discrimination between Dispharynx nasuta and Cheilospirura hamulosa (Nematoda: Acuariidae) parasitizing poultry

Autor:	Tadashi Itagaki, Peru Gopal Biswas, Yuma Ohari, Uday Kumar Mohanta
Rok vydání:	2021
Předmět:	Veterinary medicine Heterakis Nematode Acuariidae General Veterinary biology Heterakis gallinarum Multiplex polymerase chain reaction Ascaridia galli Ribosomal RNA biology.organism_classification Feces
Zdroj:	Journal of Veterinary Medical Science. 83:226-229
ISSN:	1347-7439 0916-7250
DOI:	10.1292/jvms.20-0664
Popis:	The poultry infections caused by Dispharynx nasuta and Cheilospirura hamulosa nematodes are difficult to be diagnosed by fecal examination because of their egg similarity. In this study, we analyzed DNA sequences of nuclear ribosomal 18S-ITS1-5.8S-ITS2-28S region of D. nasuta and C. hamulosa and developed conventional multiplex PCR method using species-specific primers for discriminating between the two species. The method amplified 455-bp and 319-bp fragments specific to D. nasuta and C. hamulosa, respectively, and did not produce them against the other chicken nematode species, Ascaridia galli, Oxyspirura mansoni, Heterakis gallinarum, Heterakis beramporia, and Heterakis indica, suggesting that the multiplex PCR is sensitive and available for species diagnosis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f26cc4b7a0d422c2364dae6ec17d5295 https://doi.org/10.1292/jvms.20-0664 Zobrazit plný text záznamu