Rapid detection of aspergillosis in immunocompromised patients using DIMS and chemometric analysis

Autor:	João Cleverson Gasparetto, Francinete Ramos Campos, Mário S. Piantavini, Isabela F. Zaramella, Letícia Bonancio Cerqueira, Roberto Pontarolo, Thais Martins Guimarães de Francisco
Rok vydání:	2015
Předmět:	Pathology medicine.medical_specialty General Chemical Engineering General Engineering medicine Analytical chemistry Biology Serum samples Aspergillosis medicine.disease Disease control Rapid detection Analytical Chemistry
Zdroj:	Analytical Methods. 7:6346-6351
ISSN:	1759-9679 1759-9660
Popis:	Rapid diagnosis of aspergillosis is one of the most important aspects for disease control and treatment. The purpose of this study was to develop a metabolic fingerprint of serum from immunocompromised patients that can enable rapid detection of aspergillosis. Serum samples from immunocompromised patients with and without aspergillosis were analyzed by direct infusion mass spectrometry (DIMS). Data from the mass spectra were subjected to chemometric analysis using principal component analysis and partial least squares-discriminant analysis. Our method was able to efficiently distinguish patients with and without aspergillosis and predict the presence or absence of aspergillosis in unknown samples. Therefore, DIMS along with chemometric analysis seems to be a promising technique for rapid diagnosis of aspergillosis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f269f11621b55540a4a0c48c9a9810ce https://doi.org/10.1039/c5ay01146a Zobrazit plný text záznamu