Expression of Phospholipase C-?1 in Experimental Cholesteatoma Using Mongolian Gerbils
| Autor: | Keehyun Park, Dong-Hoon Lee, Young-Myoung Chun |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Pathology
medicine.medical_specialty Phospholipase C medicine.diagnostic_test Cholesteatoma General Medicine Biology Gerbil medicine.disease Pathogenesis Immunolabeling Otorhinolaryngology Western blot embryonic structures otorhinolaryngologic diseases medicine Immunohistochemistry Signal transduction |
| Zdroj: | Acta Oto-Laryngologica. 121:477-480 |
| ISSN: | 1651-2251 0001-6489 |
| Popis: | Phospholipase C (PLC)-gamma1 is known to play a central role in ligand-mediated signal transduction for cell proliferation. The purpose of this study was to elucidate the distribution of PLC-gamma1 in deep meatal skin, retroauricular skin and cholesteatoma matrix of experimental animals. We induced canal ligation cholesteatoma using Mongolian gerbils and investigated the expression of PLC-gamma1 in this model by using Western blot analysis and immunohistochemistry. By Western blot analysis, considerably higher levels of PLC-gamma1 protein were detectable in experimental cholesteatoma. On immunohistochemistry, experimental cholesteatoma showed more intense immunolabeling of PLC-gamma1 protein than deep meatal skin and retroauricular skin. In conclusion, overexpression of PLC-gamma1 may in part contribute to abnormal proliferation and differentiation of experimental cholesteatoma in Mongolian gerbils. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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