Enterovirus 2C Protein Suppresses IKKα Phosphorylation by Recruiting IKKβ and IKKα into Viral Inclusion Bodies
Autor: | Susu He, Enhui Yang, Lianfu Ji, Deyan Chen, Yu Jin |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
biology Viral protein Chemistry Immunology Transfection medicine.disease_cause biology.organism_classification Inclusion bodies In vitro Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Virology medicine Enterovirus 71 Molecular Medicine Enterovirus Phosphorylation 030211 gastroenterology & hepatology Tumor necrosis factor alpha |
Zdroj: | Viral Immunology. 34:218-226 |
ISSN: | 1557-8976 0882-8245 |
Popis: | The nuclear factor-kappa B (NF-κB) signaling network constitutes a first line of defense against the invading viruses. However, viruses also adopted multiple strategies to interfere with NF-κB activation. Enterovirus 71 (EV71), in the family Picornaviridae, has become the main pathogen responsible for hand, foot, and mouth disease. Recent studies have reported that the nonstructural protein 2C of EV71 inhibits TNF-α induced NF-κB activation by suppressing IKKβ phosphorylation. In our study, we found that 2C can form inclusion bodies (IBs) in infected and transfected cells. Furthermore, 2C was able to sequester IKKβ into IBs through direct interaction with IKKβ. Although 2C did not directly interact with IKKα, viral protein 2C was able to sequester the IKKα into the IBs mediated by IKKβ. Our in vitro data further demonstrated that EV71 2C could suppress IKKα phosphorylation. These all together support a novel mechanism for EV71 to escape from NF-κB response, in which the phosphorylation of IKKα was suppressed by being recruited into viral IBs in the presence of 2C and IKKβ. |
Databáze: | OpenAIRE |
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