Effective Differentiation of Induced Pluripotent Stem Cells Into Dental Cells

Autor: Hidemitsu Harada, Keishi Otsu, Makiko Arakaki, Jong Min Lee, Eun Jung Kim, Satoshi Fukumoto, Han Sung Jung, David W. Green, Kyung Sik Yoon
Rok vydání: 2018
Předmět:
Zdroj: Developmental Dynamics. 248:129-139
ISSN: 1058-8388
DOI: 10.1002/dvdy.24663
Popis: Background A biotooth is defined as a complete living tooth, made in laboratory cultures from a spontaneous interplay between epithelial and mesenchymal cell-based frontal systems. A good solution to these problems is to use induced pluripotent stem cells (iPSCs). However, no one has yet formulated culture conditions that effectively differentiate iPSCs into dental epithelial and dental mesenchymal cells phenotypes analogous to those present in tooth development. Results Here, we tried to induce differentiation methods for dental epithelial cells (DEC) and dental mesenchymal cells from iPSCs. For the DEC differentiation, the conditional media of SF2 DEC was adjusted to embryoid body. Moreover, we now report on a new cultivation protocol, supported by transwell membrane cell culture that make it possible to differentiate iPSCs into dental epithelial and mesenchymal cells with abilities to initiate the first stages in de novo tooth formation. Conclusions Implementation of technical modifications to the protocol that maximize the number and rate of iPSC differentiation, into mesenchymal and epithelial cell layers, will be the next step toward growing an anatomically accurate biomimetic tooth organ. Developmental Dynamics 248:129-139, 2019. © 2018 Wiley Periodicals, Inc.
Databáze: OpenAIRE
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