| Autor: |
Doris Schiffer, Georg Gübitz, Ingrid K. Haaksman, H. B. M. Lenting, Eva Sigl, Nicole Ellen Papen-Botterhuis, Barbara Binder, Michael Schintler, Konstantin Schneider, Andrea Heinzle |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Wound Repair and Regeneration. 21:482-489 |
| ISSN: |
1067-1927 |
| DOI: |
10.1111/wrr.12040 |
| Popis: |
A gelatinase-based device for fast detection of wound infection was developed. Collective gelatinolytic activity in infected wounds was 23 times higher (p ≤ 0.001) than in noninfected wounds and blisters according to the clinical and microbiological description of the wounds. Enzyme activities of critical wounds showed 12-fold elevated enzyme activities compared with noninfected wounds and blisters. Upon incubation of gelatin-based devices with infected wound fluids, an incubation time of 30 minutes led to a clearly visible dye release. A 32-fold color increase was measured after 60 minutes. Both matrix metalloproteinases and elastases contributed to collective gelatinolytic enzyme activity as shown by zymography and inhibition experiments. The metalloproteinase inhibitor 1,10-phenanthroline (targeting matrix metalloproteinases) and the serine protease inhibitor phenylmethlysulfonyl fluoride (targeting human neutrophil elastase) inhibited gelatinolytic activity in infected wound fluid samples by 11-37% and 60-95%, respectively. Staphylococcus aureus and Pseudomonas aeruginosa, both known for gelatinase production, were isolated in infected wound samples. © 2013 by the Wound Healing Society. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
|
Nepřihlášeným uživatelům se plný text nezobrazuje |
K zobrazení výsledku je třeba se přihlásit.
|
