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Department of Plant Resources Conservation, Korea National Arboretum, Gyeonggi 487-821, Korea(Received 25, July 2012., Revised 13, July 2012., Accepted 8, September 2012)ABSTRACT: This study was performed to isolate and identify the orchid mycorrhizal fungi (OMF) from rootsof five species of terrestrial orchids in Korea; Cymbidium goeringii, Spiranthes sinensis, Calanthe discolor, Bletillastriata, Pogonia minor. DNA was extracted from isolated OMF and ITS region was amplified using primers, ITS1-OF and ITS4-OF. Four species of OMF belong to Tulasnellaceae and Sebacinaceae were identified; Tulasnellacalospora, Tulasnella irregularis, Tulasnella sp., Sebacina vermifera.KEYWORDS : Cymbidium goeringii, ITS, OMF, Orchid, Sebacinaceae, Tulasnellaceae |