The marine bacteriaShewanella frigidimarina NCIMB400 upregulates the type VI secretion system during early biofilm formation
| Autor: | Natacha Jean, Tassadit Ouidir, Yves Blache, Perrine Van Overtvelt, Maëlle Molmeret, Denis Linares, Julie Hardouin |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
biology 030106 microbiology Biofilm biology.organism_classification Agricultural and Biological Sciences (miscellaneous) Shewanella Shewanella frigidimarina 3. Good health Microbiology 03 medical and health sciences Marine bacteriophage Secretion 14. Life underwater Shewanella oneidensis Ecology Evolution Behavior and Systematics Bacteria Type VI secretion system |
| Zdroj: | Environmental Microbiology Reports. 8:110-121 |
| ISSN: | 1758-2229 |
| DOI: | 10.1111/1758-2229.12358 |
| Popis: | Shewanella sp. are facultative anaerobic Gram-negative bacteria, extensively studied for their electron transfer ability. Shewanella frigidimarina has been detected and isolated from marine environments, and in particular, from biofilms. However, its ability to adhere to surfaces and form a biofilm is poorly understood. In this study, we show that the ability to adhere and to form a biofilm of S. frigidimarina NCIMB400 is significantly higher than that of Shewanella oneidensis in our conditions. We also show that this strain forms a biofilm in artificial seawater, whereas in Luria–Bertani, this capacity is reduced. To identify proteins involved in early biofilm formation, a proteomic analysis of sessile versus planktonic membrane-enriched fractions allowed the identification of several components of the same type VI secretion system gene cluster: putative Hcp1 and ImpB proteins as well as a forkhead-associated domain-containing protein. The upregulation of Hcp1 a marker of active translocation has been confirmed using quantitative reverse transcription polymerase chain reaction. Our data demonstrated the presence of a single and complete type VI secretion system in S. frigidimarina NCIMB400 genome, upregulated in sessile compared with planktonic conditions. The fact that three proteins including the secreted protein Hcp1 have been identified may suggest that this type VI secretion system is functional. |
| Databáze: | OpenAIRE |
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