Protein tailoring of human granulocyte colony-stimulating factor

Autor:	Haruhiko Tsumura, Shigeru Matsuki, Katsuhiko Asano, Yuji Okada, Rika Ishikawa, Masaharu Ishikawa
Rok vydání:	1993
Předmět:	Chemistry fungi Mutant Chemical modification Mutagenesis (molecular biology technique) Bioengineering Biological activity General Medicine Applied Microbiology and Biotechnology law.invention Biochemistry law Recombinant DNA Site-directed mutagenesis Biotechnology Thermostability Cysteine
Zdroj:	Biotechnology Letters. 15:673-678
ISSN:	1573-6776 0141-5492
Popis:	Recombinant human granulocyte-colony stimulating factor (rhG-CSF) was modified by site-directed mutagenesis and chemical modification in order to improve its pharmacological activity and its thermostability. The mutant rhG CSF which 17th cysteine was substituted with alanine was chemically modified by activated polyethylene glycol. The chemically modified mutant rhG-CSF greatly increased both its biological activityin vivo and its thermostability. This is a successful example of protein tailoring in which site-directed mutagenesis and chemical modification were used at the same time.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f21c3cbed4b21207a7ab9c4d07872052 https://doi.org/10.1007/bf01080138 Zobrazit plný text záznamu Full text from SpringerLink