Characterization of upstream sequences of the LIM2 gene that bind developmentally regulated and lens-specific proteins*

Autor:	Robert L. Church, Heng Hsu
Rok vydání:	2004
Předmět:	Cell Methylation Biology Molecular biology Lens Fiber Cell biology AP-1 transcription factor medicine.anatomical_structure Membrane protein Transcription (biology) medicine Consensus sequence General Materials Science Gene
Zdroj:	Progress in Natural Science. 14:1039-1052
ISSN:	1745-5391 1002-0071
DOI:	10.1080/10020070412331344781
Popis:	During lens development, lens epithelial cells differentiate into fiber cells. To date, four major lens fiber cell intrinsic membrane proteins (MIP) ranging in size from 70 kD to 19 kD have been characterized. The second most abundant lens fiber cell intrinsic membrane protein is MP19. This protein probably is involved with lens cell communication and relates with cataractogenesis. The aim of this research is to characterize upstream sequences of the MP19 (also called LIM2) gene that bind developmentally regulated and lens-specific proteins. We have used the gel mobility assays and corresponding competition experiments to identify and characterize cis elements within approximately 500 bases of LIM2 upstream sequences. Our studies locate the positions of some cis elements, including a “CA” repeat, a methylation Hha I island, an FnuD II site, an Ap1 and an Ap2 consensus sequences, and identify some specific cis elements which relate to lens-specific transcription of LIM2. Our experiments also preli...
Databáze:	OpenAIRE
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