Simultaneous Determination of N-Dealkylated Metabolites of Four Butyrophenone-Type Agents by HPLC with Fluorescence Detection after Pre-Column Derivatization with Dansyl Chloride
| Autor: | Yasuhiko Higashi, Shota Nakamura, Youichi Fujii, Masatoshi Sakata |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Chromatography
Chemistry Butyrophenone Metabolite Clinical Biochemistry Dansyl chloride Pharmaceutical Science Biochemistry High-performance liquid chromatography Fluorescence spectroscopy Analytical Chemistry chemistry.chemical_compound Bromperidol medicine Derivatization Quantitative analysis (chemistry) medicine.drug |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 31:2762-2770 |
| ISSN: | 1520-572X 1082-6076 |
| Popis: | The basic metabolites of butyrophenone-type agents are sometimes more neurotoxic than the parent compounds. Recently, we developed a simple high performance liquid chromatographic (HPLC) method coupled with dual ultraviolet detection to determine the N-dealkylated basic metabolites, i.e., 1,3-dihydro-1-(1,2,3,6-tetrahydro-4-pyridinyl)-H-benzimidazole-2-one, 1-phenyl-1,3,8-triazaspiro[4.5]decan-4-one, 4-(4-chloro-phenyl)-4-hydroxypiperidine, and 4-(4-bromophenyl)-4-hydroxypiperidine (BPHP), of droperidol, spiperone, haloperidol, and bromperidol, respectively, in phosphate buffered saline (pH 7.4). An HPLC analysis with fluorescence detection was also developed to quantify these metabolites in rat plasma after precolumn derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). In this study, dansyl chloride (Dns-Cl) was used for determination of these metabolites instead of NBD-F. The samples in phosphate buffered saline were mixed with borate buffer (pH 9.5) and Dns-Cl solution in acetoni... |
| Databáze: | OpenAIRE |
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