Anti-Hepatitis B surface Antigen monoclonal antibody IgM production in suspension and immobilized cell bioreactors

Autor:	Mei-Jywan Syu, Chi Wai Kwong, Shan Shan Chou, Jih Han Hsieh
Rok vydání:	1989
Předmět:	medicine.drug_class Cell Bioengineering General Medicine Biology Monoclonal antibody Hepatitis b surface antigen Applied Microbiology and Biotechnology Suspension culture Molecular biology medicine.anatomical_structure Antigen Cell culture medicine Bioreactor Suspension (vehicle) Biotechnology
Zdroj:	Biotechnology Letters. 11:377-382
ISSN:	1573-6776 0141-5492
DOI:	10.1007/bf01089467
Popis:	We have cultivated a hybridoma cell-line H505AC in suspension and alginate immobilized cell bioreactors to produce anti-Hepatitis B surface Antigen (anti-HBsAg) monoclonal antibody IgM. The specific IgM production rates correlate linearly with the specific glucose consumption rate in suspension culture with a maximum production rate of 300 μg/106 cells/day. In alginate-cell immobilized airlift bioreactor, a total of 1143 milligrams IgM was produced in 9 days operation with a volumetric productivity 44.1 mg/day.L
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f2013269d5418921e69edb51922923d7 https://doi.org/10.1007/bf01089467 Zobrazit plný text záznamu Full text from SpringerLink