| Autor: | Johan R. Lillehaug, Holm Holmsen, Vidar A.T. Thorsen, Ove Bruland |
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| Rok vydání: | 1998 |
| Předmět: |
Phospholipase D
PLD2 Clinical Biochemistry Cell Biology General Medicine 12-O-Tetradecanoylphorbol-13-acetate chemistry.chemical_compound chemistry Biochemistry Phosphatidylcholine Choline lipids (amino acids peptides and proteins) CDP-choline pathway Choline transport Molecular Biology Protein kinase C |
| Zdroj: | Molecular and Cellular Biochemistry. 187:147-154 |
| ISSN: | 0300-8177 |
| DOI: | 10.1023/a:1006813524791 |
| Popis: | We have shown that 12-O-tetradecanoylphorbol 13-acetate (TPA) increases protein kinase C (PKC)-mediated choline transport, incorporation of choline into phosphatidylcholine (PtdCho) and PtdCho degradation by phospholipase D (PLD) in C3H10T1/2 Cl 8 cells. Dual prelabeling experiment using [3H]/[14C]choline indicated that intracellular choline generated from the PLD reaction was not directly recycled to PtdCho synthesis within the cell, and that a large fraction of the choline was transported out of the TPA-treated cells. In contrast, medium derived choline was preferably channeled to PtdCho synthesis. These results indicate that in TPA-treated cells, the choline derived from the PKC-mediated increased PLD activity and the choline newly taken up by the cell behave as two distinctly different metabolic pools. |
| Databáze: | OpenAIRE |
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