Differential expression of PSP94 in rat prostate lobes as demonstrated by an antibody against recombinant GST-PSP94
Autor: | Joseph L. Chin, Sean Jiang, Franky L. Chan, James Koropatnick, Hideki Sakai, Yushi Imasato, Jim W. Xuan, Yuzhen Guo, Joseph Kwong |
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Rok vydání: | 1999 |
Předmět: |
medicine.medical_specialty
Expression vector biology medicine.medical_treatment Cell Biology medicine.disease Biochemistry Molecular biology law.invention Prostate cancer Steroid hormone medicine.anatomical_structure Endocrinology Glutathione S-transferase Prostate law Polyclonal antibodies Internal medicine medicine biology.protein Recombinant DNA Antibody Molecular Biology |
Zdroj: | Journal of Cellular Biochemistry. 74:406-417 |
ISSN: | 1097-4644 0730-2312 |
Popis: | Prostate secretory protein (PSP94, 94 amino acids) is one of the most abundant proteins secreted from the prostate. Its biological role is unknown and still controversial, although it is assumed to have the potential to be a biomarker and a suppressor of prostate cancer. In order to establish an animal model to further elucidate its biological role, we expressed the mature form of rat PSP94 in Escherichia coli, using a glutathione S-transferase (GST) fusion expression vector; we generated a polyclonal rabbit antibody against the recombinant protein. The antibody specifically recognized recombinant rat PSP94 and cross-reacted only very weakly with its human homologue. Using the characterized anti-rat PSP94 antibody, we found that PSP94 was located primarily in rat prostate. Furthermore, PSP94 is present at different levels in different lobes of rat prostate, with significant levels detectable only in the lateral lobe (LP). In addition, the most abundant PSP94 expression was found in the prostate lobe secretions, and PSP94 levels in LP secretions were at least seven times higher than in secretions from the dorsal prostate (DP). The rat ventral prostate (VP) and other regions of the male accessory glands were found to be almost completely devoid of PSP94. Since most rat prostate dysplasia induced by steroid hormone treatment occurs only in dorsolateral prostate, prostate tissue-specific expression and the expression of PSP94 in dorsolateral, but not other, lobes of the prostate suggest a potential role in prostate targeting and prostate cancer development. J. Cell. Biochem. 74:406–417, 1999. © 1999 Wiley-Liss, Inc. |
Databáze: | OpenAIRE |
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